FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16038814
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC198131112.353226366986986TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC391890.24433851530418646TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC2179650.068.461745
CGTATGC2182350.068.45280544
TATGCCG2180600.068.4112246
TCGTATG2188150.068.2887543
ATGCCGT2186050.068.23266647
AGTCACG2212700.068.21512628
CTCGTAT2137550.068.1595242
GCCGTCT2186900.068.1380749
TGCCGTC2191800.068.0532448
GTCACGA2213900.067.95251529
ACGTCTG2237750.067.87208615
CAGTCAC2227550.067.8609127
ACACGTC2246600.067.83909613
CACGATC2212350.067.8076531
CACACGT2249450.067.78602612
CCGTCTT2195800.067.7393650
GATCTCG2135200.067.7232439
CACGTCT2244650.067.71001414
CAGATCT2131150.067.696337
ACTCCAG2232250.067.68291523
Produced by FastQC (version 0.11.5)