FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15997017
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC7047084.405246303107636TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC263360.1646306933348886TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC219490.13720683049846105TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC176570.11037682837994109TruSeq Adapter, Index 8 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC820500.066.5708144
ATGCCGT773950.066.3465947
TATGCCG808100.066.2974246
GTATGCC827850.066.2882145
TCGTATG811550.065.9422543
CTCGTAT824700.065.9342742
GTCACAC1020700.065.7617829
ACGTCTG1035200.065.6718215
CGTCTGA1035900.065.6508916
ACACGTC1039550.065.6288913
TGCCGTC772800.065.6067748
CAGTCAC1031750.065.5648627
CCAGTCA1032500.065.45560526
ACTCCAG1035300.065.410223
CACACGT1044850.065.3125312
GCACACG1046900.065.2416711
CACGTCT1043050.065.2276914
GAACTCC1042000.065.2006121
TCCAGTC1036100.065.1910225
AGTCACA1034800.065.1519828
Produced by FastQC (version 0.11.5)