FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15990833
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC5996203.749773385789221TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC704600.065.8951544
GTATGCC709150.065.5661445
TATGCCG702250.065.5181746
ATGCCGT692850.065.2903947
TCGTATG705100.065.1786843
CTCGTAT703500.065.1370542
GCCGTCT682900.064.8108749
TGCCGTC694350.064.7553448
AGTCACC792850.064.1248328
CATCTCG746050.063.45268639
CCGTCTT702450.063.4001550
TCTCGTA729250.063.24960741
CTGCTTG652600.063.08217257
CAGTCAC810500.062.98827727
GTCACCA809300.062.7351229
ACCAGAT800400.062.71220832
ATCTCGT747200.062.60593440
CCAGTCA811950.062.5644626
TCACCAG811800.062.38730630
CAGATCA799650.062.18989634
Produced by FastQC (version 0.11.5)