FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16349261
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1844021.1278919579300863TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC222050.061.68212544
GTATGCC227100.060.21784245
TATGCCG227500.060.0813846
ATGCCGT230200.059.6047747
CTCGTAT230400.059.18733242
TCGTATG232550.059.00208343
GTCACGC234500.058.60840229
GCCGTCT233850.058.5393749
TGCCGTC236600.058.0366748
AGTCACG241300.057.76899328
TCACGCC237550.057.51651830
CACGCCA237850.057.3555231
CCGTCTT248050.055.1040450
CTGCTTG247100.054.43513557
ACGCCAA252550.054.03207832
CAGTCAC262550.053.3734427
CGCCAAT253750.053.3489933
TCTCGTA257950.052.85185241
GCCAATA256450.052.8016134
TATCTCG260200.052.19289839
Produced by FastQC (version 0.11.5)