FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14683588
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC3542362.4124621311902787TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC360320.24538961458194006TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC453300.065.4640744
GTATGCC456900.065.2470445
TATGCCG456150.064.870946
ATGCCGT456650.064.3631847
TCGTATG455250.064.3073943
TGCCGTC456650.063.96438648
GCCGTCT452550.063.9087949
CTCGTAT416500.063.7432942
GATCTCG425250.063.12223439
CACAGTG494500.062.52997633
CCGTCTT465400.062.42292450
GTCACAC505150.061.592129
CTGCTTG444350.061.56711657
CAGTCAC511650.061.144827
ACACAGT508450.060.97958832
AGTGATC448650.060.90391536
CACACAG511900.060.85513731
TCTCGTA438650.060.77903441
GTGATCT448100.060.11290437
AGTCACA522600.059.9239128
Produced by FastQC (version 0.11.5)