FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16149551
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3026201.8738601463285265TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC378250.063.69275744
GTATGCC380800.063.24765445
TATGCCG381950.063.02057646
ATGCCGT385600.062.4514647
TCGTATG386550.062.38833243
GCCGTCT387250.062.08536549
CTCGTAT374150.061.9486642
TGCCGTC389700.061.84830548
ACTGACC398700.060.34010732
CCGTCTT402100.059.79285450
CTGCTTG401700.059.28473757
CTGACCA404950.059.18428833
CAGTCAC418800.058.73069827
GTCACTG419350.058.29480429
TCTCGTA396500.058.1650541
CACTGAC418650.057.9241331
GACCAAT410300.057.62889535
CCAGTCA429400.057.1579326
CCAATCT400000.057.0321837
TGCTTGA421100.056.59523458
Produced by FastQC (version 0.11.5)