FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12973038
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1827901.4089991873915733TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC219600.062.28729244
TATGCCG219100.062.2861446
CACTTAG230700.061.92544631
GTATGCC222950.061.57139645
ACTTAGG230800.061.54984732
ATGCCGT221250.061.5068647
GCCGTCT221350.060.95749349
TGCCGTC223700.060.6924148
TCGTATG224050.060.65985543
CTCGTAT222050.060.51219642
GGCATCT227700.059.80884637
AGGCATC230950.059.49531636
TTAGGCA243200.058.00890434
CTGCTTG223850.057.3199657
CCGTCTT237000.057.1835350
GCATCTC242200.056.1566138
TCTCGTA240350.056.1376441
CATCTCG246300.055.20738239
CAGTCAC264000.054.77745427
ATCTCGT249750.054.34707640
Produced by FastQC (version 0.11.5)