FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15403662
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC4599252.985815970254346TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC518000.065.868944
GTATGCC523550.065.4106945
TATGCCG520300.065.3479346
ATGCCGT520200.064.8018247
CTCGTAT524750.064.68725642
TCGTATG525000.064.6833343
GCCGTCT514050.064.6504549
TGCCGTC521850.064.3418448
CGATGTA566650.063.56759634
GTCACCG577550.063.4931729
AGTCACC580800.063.3487928
CCGATGT570700.063.2323933
GTATCTC552550.063.1922738
CCGTCTT536350.062.44577450
TCTCGTA548950.062.3900941
CTGCTTG502950.062.2564957
ACCGATG585500.062.0998832
CAGTCAC595400.061.94761327
GATGTAT581500.061.5472135
ATGTATC575300.061.4940536
Produced by FastQC (version 0.11.5)