FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15189243
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2786911.8347918984507654TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC333450.063.7600444
ATGCCGT335450.063.37989447
TATGCCG337450.062.95239646
GTATGCC338850.062.733245
TGCCGTC339550.062.5832648
CTCGTAT332250.062.31503342
GCCGTCT343000.061.88193549
TCGTATG347150.061.37508443
CACGATC334500.060.85450736
CGATCTC336650.060.7287838
ACGATCT337900.060.39933837
CATCACG352300.060.10235633
GATCTCG342450.059.82327339
CTGCTTG357100.059.22134457
CCGTCTT359800.058.9724650
TCACGAT356550.058.60241735
CAGTCAC372650.058.2178827
TCTCGTA355450.057.88208441
CCAGTCA376800.057.57592826
ATCACGA366700.057.5047234
Produced by FastQC (version 0.11.5)