FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17538842
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT3339721.9041850083374945TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC390800.063.37711346
TATGCCG392900.062.8503948
ATGCCGT393700.062.811449
GTATGCC395250.062.53055247
TGCCGTC395700.062.45059650
GCCGTCT400150.061.83411851
CTCGTAT400750.061.63813444
TCGTATG402800.061.42853545
CCGTCTT415850.059.48262852
CTGCTTG418700.058.758459
TATCTCG426350.058.1321541
GTCACAC432600.058.02260229
CACACTG431300.057.9701231
TCTCGTA433350.057.17052543
ATCTCGT443250.056.01143342
CAGTCAC451800.055.97402627
CCAGTCA455400.055.4157926
GATATAT451600.055.1039437
TGATATA453200.054.98568336
AGTCACA459300.054.86237328
Produced by FastQC (version 0.11.5)