FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17407223
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT4836242.7782949641077153TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC553100.065.2270646
GTATGCC554900.064.9522147
ATGCCGT555300.064.84869449
TATGCCG557000.064.6317448
TGCCGTC560250.064.31248550
GCCGTCT559250.064.23308651
TCGTATG562850.063.9908945
CTCGTAT561900.063.9867744
CGAGTGG570500.063.19286333
ACGAGTG572150.063.10849832
CCGTCTT578950.062.1020152
CACGAGT583800.062.04625331
GGATATC580350.062.0151638
GTGGATA578250.062.00823236
AGTCACG592500.061.69811228
CTGCTTG579300.061.50116359
TATCTCG586750.061.3283841
GTCACGA596250.061.13984729
TCTCGTA591500.061.01516343
GAGTGGA593700.060.60575534
Produced by FastQC (version 0.11.5)