FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15609166
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT2453141.5716022239753231TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT277000.064.4765434
CCGTACG279150.064.2929833
CGTATGC286700.062.70255746
GTACGTA288150.062.19984435
GTATGCC289800.062.0801447
TATGCCG289000.062.0218748
ATGCCGT289000.061.8888449
TGCCGTC292750.061.11998450
GCCGTCT291100.061.11694751
CTCGTAT292250.061.01978344
TCGTATG294050.060.9791545
CACCGTA298400.060.46148731
TACGTAA296700.060.4319936
GTCACCG303250.059.85246329
CGTAATC299050.059.57203338
ACCGTAC304550.059.29838632
ACGTAAT304300.058.9692537
AGTCACC311100.058.55520628
CCGTCTT307050.058.05690852
GTAATCT309950.057.25122539
Produced by FastQC (version 0.11.5)