FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17673881
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT9830235.562009838133458TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGAT753440.42630138790682137TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTTT580410.3283998573940834TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGTAT452970.25629345359969324TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGGAT242010.13693087556717168TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGTT179080.10132466095024631TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG1498650.067.3644928
CGTATGC949450.067.255646
GTATGCC957600.067.1837847
GTCACGT1492350.067.15443429
TATGCCG927700.067.0705748
CGGAATC1460250.066.7596138
CTCGTAT1075950.066.7078544
CAGTCAC1517700.066.66402427
ATGCCGT936700.066.5941249
ACTCCAG1521800.066.4997623
TCGTATG998600.066.4975245
GTTTCGG1486050.066.4887234
CCAGTCA1520500.066.4764326
CGTTTCG1491600.066.3587433
TCCAGTC1524100.066.31290425
ACGTCTG1531950.066.2813615
TGCCGTC955100.066.2455350
GGAATCT1325750.066.22768439
GAACTCC1530800.066.2038721
GCCGTCT906800.066.19485551
Produced by FastQC (version 0.11.5)