FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17374353
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT14272368.21461380461189TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTTA1551450.068.3970436
CGTATGC1567900.068.0076246
GTATGCC1572350.067.8572547
TATGCCG1568850.067.854548
GCCTTAT1562350.067.81389637
CTCGTAT1568900.067.6906444
CGTGGCC1577250.067.6855633
ATGCCGT1572250.067.676449
TGGCCTT1565600.067.58398435
TCGTATG1576950.067.5380145
AGTCACG1611700.067.528328
TGCCGTC1576100.067.52807650
GCCGTCT1568250.067.4728951
GTGGCCT1572750.067.4145234
GTCACGT1606100.067.3497229
CAGTCAC1629050.066.9184727
CCTTATC1579900.066.8460938
CCGTCTT1585400.066.80942552
CCAGTCA1630550.066.8085326
CTGCTTG1550150.066.7765359
Produced by FastQC (version 0.11.5)