FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16193821
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT13286348.204573830969233TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAATGATCTCGTAT208450.1287219365954459TruSeq Adapter, Index 19 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1537300.067.7265346
TATGCCG1536600.067.6815848
GTATGCC1537250.067.67615547
AGTCACG1553700.067.64902528
ATGCCGT1539300.067.51221549
CTCGTAT1526400.067.4044344
GTCACGT1550250.067.3852229
TCGTATG1543350.067.33053645
TGCCGTC1543450.067.31254650
GATCTCG1513600.067.2989841
GCCGTCT1543150.067.2839751
ACGATCT1481950.067.2491939
CGATCTC1488250.067.2052440
ACGTCTG1568900.067.2042515
ACACGTC1578250.067.12742613
GCACACG1585950.067.1194811
CGTCTGA1572600.067.052616
CACGTCT1575850.067.0322214
GAACTCC1558850.067.0254621
AACGATC1490000.066.9555138
Produced by FastQC (version 0.11.5)