FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM107.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM107.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16197383
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT8692355.366515072218765TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC991500.067.0431233
ACGTCCG998050.067.0340432
TCCGCAC985550.066.7695935
CGTATGC1011100.066.7683946
TATGCCG1011250.066.7063648
GTATGCC1011950.066.6848447
GTCCGCA992400.066.6686534
AGTCACG1027800.066.5733428
CACGTCC1008050.066.569431
ATGCCGT1016750.066.32059549
CGCACAT994050.066.26800537
CTCGTAT1011600.066.2542244
TCGTATG1017400.066.21023645
GCCGTCT1015600.066.1880951
TGCCGTC1019700.066.1012750
GTCACGT1031250.066.08333629
CCGCACA995400.066.03937536
TCACGTC1028100.065.8670930
CAGTCAC1039800.065.7282327
GCACATC1007150.065.5503738
Produced by FastQC (version 0.11.5)