FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17462117
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT9701975.556010190516992TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG1082850.067.70306435
GTCCCGA1086600.067.47417436
CACCCGT1102200.067.3177531
ACCCGTC1100250.067.2787832
CGTATGC1112800.067.224546
TCACCCG1106400.067.187130
CCGTCCC1093550.067.1674734
GTCACCC1109900.067.1614729
CCCGATC1076600.067.1506938
GTATGCC1113900.067.0671847
CCCGTCC1102000.066.9849533
TATGCCG1117500.066.8626948
TCGTATG1117850.066.70769545
ATGCCGT1119850.066.7069549
AGTCACC1129400.066.7018228
CTCGTAT1105350.066.5059344
ACGTCTG1148900.066.4595815
GCCGTCT1122100.066.4386851
TGCCGTC1125000.066.4241250
GCACACG1160000.066.24769611
Produced by FastQC (version 0.11.5)