FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13801292
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT5385903.902460726140712TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGAT245010.17752685763043055TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTTT233020.16883926519343262TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGTAT138540.1003819062737025TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC774950.066.0868613
ACGTCTG773100.066.0186615
GCACACG777450.065.9416311
CGTATGC574100.065.9001946
GTATGCC578000.065.746247
CACGTCT776900.065.66421514
ATGCCGT563150.065.5892549
CACACGT781500.065.5683112
TATGCCG565400.065.5022948
TCGTATG593600.065.26182645
CGTCTGA782250.065.2198316
CAGTCAC777650.065.105627
GATCGGA788100.065.097061
ACTCCAG776350.064.9692923
TGCCGTC575400.064.94810550
GAACTCC779700.064.931621
CCAGTCA777650.064.79838626
GCCGTCT551900.064.7575351
AGCACAC794500.064.6767610
TCCAGTC778500.064.6651725
Produced by FastQC (version 0.11.5)