FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16288231
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT12545807.702371116912573TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1473250.067.46033513
ACGTCTG1468600.067.41460415
CACGTCT1471750.067.3392814
GCACACG1478400.067.3338211
CGTATGC1400700.067.3141246
CACACGT1480350.067.174212
CGTCTGA1473650.067.1574616
CCGTATC1422550.067.1513838
GTATGCC1407200.067.12775447
TATGCCG1401100.067.1056648
CGTATCT1412550.067.035239
ATGCCGT1404650.066.9503949
CTCGTAT1406550.066.91180444
GCCGTCT1392500.066.8732851
TCGTATG1406400.066.8540245
TGCCGTC1408150.066.78895650
CAGTCAC1473350.066.773427
GTTCCGT1430150.066.76494635
ACTCCAG1473950.066.7022523
CCAGTCA1472700.066.6877826
Produced by FastQC (version 0.11.5)