FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19023641
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT238654212.545137915502085TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2638900.067.54314446
GTATGCC2641600.067.45087447
TATGCCG2640650.067.41530648
CTCGTAT2615300.067.4086844
TCGTATG2641800.067.3285545
CACAGTC2665150.067.300731
ATGCCGT2647200.067.2683349
GTCACAG2676100.067.2186329
GCCGTCT2647700.067.1845151
CAGTCAC2698350.067.1331927
CCAGTCA2695750.067.1048726
TGCCGTC2654000.067.09167550
AGTCAAC2650150.067.0840134
ACTCCAG2699300.067.07944523
ACGTCTG2723700.067.0698715
TCCAGTC2695550.067.0189725
GAACTCC2714600.067.0029421
CACGTCT2730750.066.9953914
ACAGTCA2672650.066.9811632
ACACGTC2740150.066.9631813
Produced by FastQC (version 0.11.5)