FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM102.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM102.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15981996
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC200999912.57664562048445TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC231050.14456892618418876TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC203770.127499719058871TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2254650.068.09379644
GTATGCC2257250.068.07139645
CTCGTAT2197400.068.0183642
TCGTATG2251050.067.9974543
TATGCCG2254050.067.98608446
GCCGTCT2236200.067.8669449
ATGCCGT2252650.067.8478747
CCTTGTA2279000.067.8333433
AGTCACC2317650.067.7695828
GTCACCT2305950.067.759829
TGCCGTC2251700.067.73151448
CACCTTG2300000.067.6618331
GTAATCT2212600.067.615437
ACCTTGT2290450.067.57175432
ACGTCTG2350850.067.5466615
CAGTCAC2336900.067.5333227
TCTCGTA2217200.067.4890241
CGTCTGA2353100.067.47336616
CCGTCTT2249850.067.4709650
CCAGTCA2332750.067.45586426
Produced by FastQC (version 0.11.5)