FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15362045
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC8618355.610158022580978TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC954450.067.675445
CGTATGC955400.067.65639544
ACGGCTA959000.067.6529732
CGGCTAC955800.067.4805733
TATGCCG956700.067.4540446
GTCACGG969000.067.3990529
AGTCACG974050.067.3653628
CACGGCT965250.067.3091931
GGCTACA956950.067.1452234
TCGTATG962600.067.1389943
TCACGGC969950.067.1275930
ATGCCGT960900.067.1225747
GCCGTCT960500.067.03281449
CTCGTAT955150.066.9778342
GCTACAT953650.066.96696535
TGCCGTC964300.066.8024148
CAGTCAC986400.066.472927
ACGTCTG991850.066.33089415
CCGTCTT973250.066.1013550
ACTCCAG985350.066.0629523
Produced by FastQC (version 0.11.5)