FastQCFastQC Report
Mon 31 Aug 2020
H5CCJBGXG_n01_AKM100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5CCJBGXG_n01_AKM100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14791055
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC9835636.6497149797631065TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC242660.16405861515625492TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATTC223130.150854688864317TruSeq Adapter, Index 10 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1095500.067.46590444
GTATGCC1099700.067.42786445
CACTAGC1254350.067.29156531
TATGCCG1082000.067.286346
TCGTATG1080050.067.2182343
ACTAGCT1251200.067.0978132
ATGCCGT1059950.066.9879647
CTCGTAT1104400.066.98379542
TGCCGTC1055050.066.7779748
GCCGTCT1032750.066.734749
TAGCTTA1252250.066.6851734
GCTTATC1225200.066.647936
TCACTAG1269250.066.6125930
CTAGCTT1255100.066.5941933
CAGTCAC1279200.066.4713427
ACGTCTG1288850.066.2375215
CCAGTCA1282800.066.1915926
ACACGTC1296450.066.086113
CCGTCTT1065050.066.0545550
TCTCGTA1134550.066.0386941
Produced by FastQC (version 0.11.5)