FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n02 mk277.3420000000f382.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n02 mk277.3420000000f382.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5058492
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT4220218.342822327286472Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGA462400.044.708718
TGTAGGG462200.044.68423517
GTGTAGG463900.044.6126316
TAGGGAA463150.044.60715519
GAGTGTA465500.044.3916327
TCGTGTA462800.044.38806514
CGTGTAG465450.044.28653715
TAGATCT466650.044.25723632
AGTGTAG467050.044.2307328
AGAGTGT467600.044.14415426
GTGTAGA468850.044.1189429
AGAGCGT470900.044.0511058
GTAGATC471000.043.97704331
TGTAGAT472100.043.8784730
GTCGTGT470700.043.75910613
AAGAGTG471950.043.7515725
GAGCGTC474150.043.687479
TCGGAAG479550.043.6694873
AAGAGCG477650.043.6692737
AAAGAGT470600.043.63326324
Produced by FastQC (version 0.11.2)