FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n02 mk276.3420000000f368.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n02 mk276.3420000000f368.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6403862
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT117521118.351597832682838Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGG105950.16544703805297492Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGACGT67020.10465559688825274Illumina Single End PCR Primer 1 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGG1291900.044.81356417
GTAGGGA1293850.044.80862818
TAGGGAA1295900.044.74989719
GTGTAGG1294450.044.74475516
TCGTGTA1285000.044.70262514
GAGTGTA1297300.044.63675727
AGAGCGT1302200.044.5856068
CGTGTAG1295400.044.58550615
AGAGTGT1298200.044.57114826
TAGATCT1299900.044.56513232
AGTGTAG1300750.044.5045328
GTGTAGA1303900.044.49399629
AAGAGTG1301450.044.4470525
TGTAGAT1306100.044.44660630
GTCGTGT1295250.044.4374613
GTAGATC1306850.044.4193831
AAGAGCG1313550.044.418247
GAGCGTC1303850.044.38949
AAAGAGT1298050.044.36691324
TCGGAAG1320000.044.363883
Produced by FastQC (version 0.11.2)