FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n02 mk275.3420000000f34f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n02 mk275.3420000000f34f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5525676
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT2359664.270355337518884Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGG256900.044.6374817
GTAGGGA257100.044.57650418
GTGTAGG258950.044.33623516
TAGGGAA258850.044.2490619
GAGTGTA260900.044.0668227
AGTGTAG261000.044.01545328
TAGATCT260850.043.98980732
GTGTAGA262400.043.8063429
CGTGTAG261200.043.79224815
AGAGTGT262150.043.77826326
TCGTGTA260200.043.7463614
GTAGATC263650.043.53116631
TGTAGAT264550.043.48434430
AGAGCGT266500.043.2706078
AAGAGTG267200.042.916825
TCTCGGT268400.042.50167536
AAGAGCG273400.042.4422957
AAAGAGT269550.042.3912524
CTCGGTG270500.042.1800437
GTCGTGT271050.042.13709613
Produced by FastQC (version 0.11.2)