FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n02 mk274.3420000000f325.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n02 mk274.3420000000f325.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7042070
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT6733669.562046386928843Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGG733150.044.63709317
GTAGGGA738550.044.62448518
TAGGGAA739650.044.5185819
GTGTAGG735750.044.49158516
GAGTGTA738600.044.38849627
TAGATCT741400.044.30949432
AGTGTAG740600.044.28077728
CGTGTAG737850.044.22532715
TCGTGTA735400.044.22315214
GTGTAGA743050.044.213529
AGAGTGT741400.044.18988826
TGTAGAT745150.044.0982730
GTAGATC747650.044.06848531
AGAGCGT746450.044.016188
AAGAGTG749500.043.7477125
TCTCGGT744100.043.60933336
AGCGTCG751600.043.6038110
AAAGAGT748950.043.602324
GAGCGTC751050.043.596799
AAGAGCG757100.043.5696957
Produced by FastQC (version 0.11.2)