FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n02 mk273.3420000000f30b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n02 mk273.3420000000f30b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8361329
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT165669919.813823855035487Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGG180560.21594653194486188Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGACGT101160.12098555145958258Illumina Single End PCR Primer 1 (98% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTAGCCGT94090.11252995785717797Illumina Single End PCR Primer 1 (98% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGA91620.10957588201588528Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCAGT84830.10145516340763532Illumina Single End PCR Primer 1 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGA1862850.044.84174718
TGTAGGG1844650.044.84145717
TAGGGAA1865350.044.7732119
GTGTAGG1848950.044.7712416
TCGTGTA1837400.044.67136414
GAGTGTA1864800.044.62970427
AGAGCGT1873100.044.5684668
TAGATCT1871100.044.56186332
AGAGTGT1865850.044.55582426
CGTGTAG1849800.044.54942315
GTGTAGA1872900.044.52584529
GTAGATC1880850.044.49115431
TGTAGAT1876150.044.46430630
AGTGTAG1871350.044.44009828
GTCGTGT1853850.044.4268113
AGCGTCG1872000.044.4239710
AAGAGTG1873050.044.39843425
AAGAGCG1890250.044.396757
GAGCGTC1874050.044.382589
GCGTCGT1860250.044.304211
Produced by FastQC (version 0.11.2)