FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n02 mk272.3420000000f2e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n02 mk272.3420000000f2e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5084805
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT779551.5330971394183257Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGG85700.042.7110917
GTAGGGA86600.042.6568918
GTGTAGG87150.042.052516
TAGGGAA88500.041.7156719
AGTGTAG89150.041.38831728
TAGATCT89450.041.35215432
GAGTGTA89900.041.14232627
GTGTAGA91400.040.4198829
CGTGTAG91050.040.30145615
AGAGTGT91800.040.21727826
TCGTGTA91650.039.79605514
TGTAGAT93700.039.57179330
GTAGATC94050.039.47237431
AAGAGTG95800.038.58426725
AGAGCGT96350.038.3926478
GGTGGTC98600.037.37889540
AAAGAGT100100.036.81442324
CGGTGGT100300.036.7453539
TCTCGGT100500.036.64983736
GTGGTCG100800.036.58540341
Produced by FastQC (version 0.11.2)