FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n02 mk271.3420000000f2c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n02 mk271.3420000000f2c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7609980
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT118890.15622905710658896Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGG16450.036.51635717
GTAGGGA16750.035.86233518
AGTGTAG16850.034.84999528
TAGGGAA17200.034.5316719
GTGTAGG19200.031.52071216
TAGATCT19150.031.01763332
GAGTGTA18900.030.95092427
GTGTAGA21850.027.18425629
AGAGTGT22450.026.5574226
TCGTGTA23650.025.78272214
CGTGTAG24350.025.0392315
TGTAGAT23700.024.96734230
GTAGATC24350.024.48598331
AGAGCGT27650.021.64648
AAGAGTG29500.020.28678125
AAAGAGT33850.017.67941724
AAGAGCG35750.016.8050637
TCTCGGT36700.016.36951636
GTCGTGT38300.015.92097813
GGTGGTC38950.015.53944440
Produced by FastQC (version 0.11.2)