FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n02 mk270.3420000000f2ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n02 mk270.3420000000f2ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5020670
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT336450.6701296838868119Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGA42850.042.0568618
TGTAGGG42500.042.03265417
AGTGTAG42850.041.69057528
TAGGGAA43550.041.32920519
TAGATCT43450.041.27021432
GTGTAGG43450.041.26898216
GAGTGTA43750.041.09007327
GTGTAGA44750.040.22213729
AGAGTGT46200.038.7645726
CGTGTAG46500.038.5628615
GTAGATC46600.038.52877431
TGTAGAT46700.038.3499130
TCGTGTA47100.037.83352314
AAGAGTG49800.036.14230325
AGAGCGT50500.035.688018
AAAGAGT52900.034.02432624
TCTCGGT55050.032.7379936
AAGAGCG55450.032.5836337
GTCGTGT55250.032.41618713
TCGGAAG58400.031.1365533
Produced by FastQC (version 0.11.2)