FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n02 mk269.3420000000f283.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n02 mk269.3420000000f283.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4240859
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1402663.3074902985456482Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGA156650.044.4193118
TGTAGGG157350.044.27889317
TAGGGAA157650.044.13755419
GTGTAGG158200.044.06942716
AGTGTAG157550.043.9968128
GAGTGTA158100.043.95552427
TAGATCT158350.043.83498832
GTGTAGA159350.043.6556529
AGAGTGT159800.043.44567526
TCGTGTA160000.043.20165314
TGTAGAT160900.043.19315730
CGTGTAG160950.043.1632115
GTAGATC161400.043.0469331
AGAGCGT163300.042.8394558
AAGAGTG163700.042.4513525
AAAGAGT165500.041.7041924
AAGAGCG168900.041.699347
TCGGAAG169600.041.63733
GTCGTGT166950.041.5254813
GAGCGTC168650.041.4004259
Produced by FastQC (version 0.11.2)