FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n02 mk268.3420000000f269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n02 mk268.3420000000f269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16974320
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT3760932.2156587126906997Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGG434500.044.39507317
GTAGGGA437450.044.3065518
GTGTAGG440100.043.830316
AGTGTAG446150.043.5859428
GAGTGTA448550.043.36752327
TAGGGAA448150.043.21856719
TAGATCT449700.043.21299732
GTGTAGA452450.043.07352429
CGTGTAG449000.042.8976315
AGAGTGT454050.042.7873226
TGTAGAT458350.042.5044730
GTAGATC459100.042.45060731
TCGTGTA452300.042.31442614
AGAGCGT459600.042.2742428
AAGAGTG474500.040.89513425
AGCGTCG475150.040.71080810
AAGAGCG481550.040.6465847
GGTGGTC468700.040.55059440
GAGCGTC479750.040.3579839
TCTCGGT475600.040.30291736