FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n02 mk267.3420000000f240.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n02 mk267.3420000000f240.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11223243
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1835491.6354363885732495Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGAA222100.043.435519
GTAGGGA223650.043.19482818
GAGTGTA224200.043.18071727
TGTAGGG223350.043.10175317
TCGTGTA221350.042.7541914
AGTGTAG226300.042.75037828
TAGATCT227750.042.39023632
AGAGTGT228500.042.3181426
GTGTAGG227700.042.25876216
GTGTAGA233450.041.59523429
CGTGTAG229900.041.5811115
AAGAGTG233800.041.3488425
TGTAGAT235950.041.22144730
GTCGTGT230750.041.17920713
GTAGATC239750.040.54968631
AAAGAGT237200.040.499724
AGGGAAA241650.040.21939520
AGAGCGT240150.040.1767358
TCGGAAG244800.040.154093
AGATCTC240100.039.81659733
Produced by FastQC (version 0.11.2)