FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n02 mk266.3420000000f226.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n02 mk266.3420000000f226.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11358346
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT3332812.9342388407607936Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGA376200.043.66257518
TAGGGAA376700.043.58072719
TGTAGGG376400.043.4899417
AGTGTAG380150.043.223228
GAGTGTA380600.043.20089327
GTGTAGG380800.043.09377716
TCGTGTA378150.042.9955614
TAGATCT383200.042.8859932
AGAGTGT386550.042.51752526
CGTGTAG385600.042.4071415
GTGTAGA388600.042.3933329
TGTAGAT391450.042.090830
GTCGTGT389400.041.89821213
AAGAGTG392950.041.86474625
GTAGATC394100.041.78530531
AGAGCGT394950.041.719638
AGGGAAA399700.041.20241520
AAAGAGT397850.041.19590424
GGGAAAG401350.041.0890921
TCGGAAG404950.041.0436023
Produced by FastQC (version 0.11.2)