FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n02 mk265.3420000000f20c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n02 mk265.3420000000f20c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10789713
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT3411563.161863526861187Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGG382450.043.24793617
GAGTGTA382700.043.24460227
GTAGGGA383100.043.15694418
TAGGGAA383750.043.09556619
GTGTAGG384700.043.02443316
TCGTGTA381650.043.00856814
AGTGTAG384750.042.99139828
TAGATCT386350.042.85630432
AGAGTGT387550.042.6679926
GTGTAGA390000.042.4707529
CGTGTAG388300.042.4069515
TGTAGAT392850.042.2206930
AAGAGTG393700.042.086825
GTCGTGT391600.042.06001313
GATCGGA398700.041.92051
GTAGATC396800.041.8467331
AAAGAGT396200.041.65637224
AGAGCGT399700.041.5735748
TCGGAAG402450.041.4901773
AGATCTC399350.041.3207433
Produced by FastQC (version 0.11.2)