FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n02 mk264.3420000000f1e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n02 mk264.3420000000f1e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4200606
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT958212.2811232474552483Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA112500.043.23591627
TAGGGAA112950.043.1816319
TGTAGGG112400.043.1327317
GTAGGGA112900.043.12104818
AGTGTAG112850.043.0624628
TAGATCT112850.042.98476432
TCGTGTA112550.042.7630714
AGAGTGT114100.042.64934526
GTGTAGA115600.042.1353629
AAGAGTG115750.042.0603225
TGTAGAT116350.041.92176430
CGTGTAG115850.041.63562415
GTGTAGG116550.041.5196916
GTCGTGT116450.041.5052913
GTAGATC117800.041.25492531
TCGGAAG119400.041.0297623
AAAGAGT118350.041.00275824
AGATCTC118100.040.98013733
AGAGCGT119900.040.7614258
GGGAAAG119950.040.75542421
Produced by FastQC (version 0.11.2)