FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n02 mk263.3420000000f1c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n02 mk263.3420000000f1c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11718525
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1670711.4256999067715432Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGA190150.043.64589318
TGTAGGG191100.043.37005217
AGTGTAG192500.043.09227428
GAGTGTA192900.042.9792227
TAGGGAA193800.042.85869619
TAGATCT194350.042.53322232
GTGTAGG195700.042.3739816
GTGTAGA199000.041.7644129
TCGTGTA198950.041.33650614
AGAGTGT200500.041.28205526
CGTGTAG200550.041.22723815
TGTAGAT202950.040.87447430
GTAGATC202950.040.8306531
AGAGCGT205050.040.283578
AAGAGTG206950.039.94020525
AAAGAGT211100.039.11189724
GTCGTGT214500.038.4247513
AAGAGCG221050.037.6023757
TCGGAAG224900.037.2623183
AGATCTC222850.037.07398233
Produced by FastQC (version 0.11.2)