FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n01 mk277.3410000000f385.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n01 mk277.3410000000f385.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5058492
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC4221348.34505619461294TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCAACTCGTATGCC73550.14539906359444674TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATATCGTATGCC61200.12098467290251719TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT484300.044.1762812
AGCACAC487500.044.00629810
GCACACG486900.044.00045811
GATCGGA493550.043.947491
CACGTCT486550.043.9072514
TCGGAAG493850.043.819243
ATCGGAA495750.043.7410052
ACACGTC489250.043.70173613
AGAGCAC492100.043.690968
ACGTCTG488650.043.69093315
CGTCTGA485150.043.6258516
GAGCACA493900.043.5135049
TCTGAAC487000.043.46012518
GTCTGAA487300.043.45645517
GTAGCAT459250.043.32307435
CTGAACT488400.043.30790719
GTATGCC481600.043.2751245
GGTAGCA479750.043.18362834
TAGCATC455600.043.04297636
CAGTCAC489800.043.04171827
Produced by FastQC (version 0.11.2)