FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n01 mk276.3410000000f36b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n01 mk276.3410000000f36b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6403862
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC114104717.81810726089975TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCGTATGCC302700.4726835150413922TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGAACTCGTATGCC277460.43326979875581334TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGACCTCGTATGCC133360.2082493345421872TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGAGCTCGTATGCC123130.19227459929648702TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1340300.044.587791
CACACGT1331150.044.50168612
TCGGAAG1342300.044.4590573
ATCGGAA1344000.044.445862
AGCACAC1336300.044.4194210
GCACACG1335450.044.40051311
CACGTCT1334500.044.3731114
AGAGCAC1341850.044.3061188
CGTCTGA1335650.044.28100216
ACGTCTG1336250.044.27964415
GAGCACA1342600.044.2595869
ACACGTC1338650.044.24731413
GTCTGAA1337200.044.22799317
CGGAAGA1349650.044.1835984
TCTGAAC1340450.044.06369418
CTGAACT1341850.043.9908919
GTATGCC1318450.043.8979245
AAGAGCA1356650.043.869217
CAGTCAC1332150.043.86363627
AACTCCA1342500.043.795322
Produced by FastQC (version 0.11.2)