FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n01 mk275.3410000000f342.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n01 mk275.3410000000f342.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5525676
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC2270704.109361460932562TruSeq Adapter, Index 22 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATATCGTATGCC69550.12586695274931067TruSeq Adapter, Index 22 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT271850.043.4758112
GCACACG275650.042.9254411
AGCACAC276300.042.89774710
CCGTACG264350.042.88788633
CACGTCT274850.042.87847514
CGTACGA264250.042.84451734
GATCGGA279450.042.813911
ACCGTAC266350.042.78547732
AGTCACC269650.042.77083628
GTATGCC266800.042.74704445
CACCGTA267400.042.69319531
ACGTCTG276650.042.57509215
GTCTGAA277200.042.54743617
GTACGAT252850.042.54273235
CGTCTGA276700.042.5104816
ACACGTC277750.042.45508213
CGTATGC269500.042.33547244
TCGGAAG283600.042.1317023
ATCGGAA284300.042.1027152
TCTGAAC280050.041.96179218