FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n01 mk274.3410000000f328.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n01 mk274.3410000000f328.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7042070
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG6317488.971055385703352TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAACTCGTATG167590.23798400186308857TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTATG160660.2281431454103694TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAATGATCTCGTATG89820.12754772389368468TruSeq Adapter, Index 19 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGACCTCGTATG73690.10464252698425322TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT766150.044.2790412
GCACACG769150.044.1472911
AGCACAC772950.043.98846410
CACGTCT767400.043.93718314
CGTCTGA767100.043.92503416
ACGTCTG767300.043.9165215
GTCTGAA772250.043.83896317
ACACGTC771850.043.73925413
GATCGGA787100.043.6668471
AGAGCAC783550.043.666178
TCGTATG763200.043.6542345
CACGTGA759650.043.6183331
TCGGAAG787800.043.6056023
TCACGTG762150.043.59037830
TCTGAAC773300.043.57868218
CTGAACT775150.043.4456519
ACGTGAA764800.043.40698232
GAGCACA788450.043.3947949
CAGTCAC768450.043.39111727
GTCACGT768550.043.38547529
Produced by FastQC (version 0.11.2)