FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n01 mk273.3410000000f30e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n01 mk273.3410000000f30e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8361329
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG164292819.6491251570175TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACAACTCGTATG292280.34956165461256217TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATATCGTATG198700.23764164763759443TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACACCTCGTATG161410.19304347430892865TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACAGCTCGTATG130820.1564583811975345TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGTCCGCACATCTCGTATG107230.12824516294000632TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGTCCGCACATCTCGTATG96450.11535247566505277TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCACATCTCGTATG84590.10116812769835992TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG84370.10090501163152413TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1978950.044.4292141
AGCACAC1956250.044.34191510
GCACACG1955300.044.34044311
TCGGAAG1980300.044.3267863
CACACGT1956750.044.32483712
ATCGGAA1984000.044.3189622
AGAGCAC1972550.044.1534358
CGTCTGA1961350.044.11878616
TCGTATG1940300.044.1077145
GAGCACA1973000.044.1034559
ACACGTC1966150.044.08202713
CGGAAGA1987750.044.0768854
GTCTGAA1969900.044.0666417
TCTGAAC1959550.044.04793518
CTGAACT1960900.043.97630319
ACGTCTG1969950.043.9467415
CACGTCT1969750.043.929514
CCGCACA1913050.043.7860836
CGCACAT1855050.043.76393537
GTCACGT1955100.043.7385129
Produced by FastQC (version 0.11.2)