FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n01 mk272.3410000000f2e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n01 mk272.3410000000f2e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5084805
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG760161.4949639170037003TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT95200.039.9407212
TATCTCG88800.039.5508741
GTCACAG93300.039.33131429
ACGTCTG96200.039.29165315
GCACACG97150.039.13902311
CTCGTAT94150.039.02401444
AGTCACA94250.038.9587428
CACGTCT97100.038.95063814
CGTCTGA97200.038.7948316
CGTATCT90250.038.61627239
TCGTATG96550.038.52041245
GTATCTC91550.038.4857140
TCACAGT95650.038.482630
AGCACAC99150.038.4176110
GTCTGAA99450.037.9849917
CCGTATC92600.037.56337438
CAGTCAC98500.037.506227
GATCGGA102150.037.4902461
ACACGTC101650.037.33995413
TCTGAAC101400.037.21013318
Produced by FastQC (version 0.11.2)