FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n01 mk271.3410000000f2c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n01 mk271.3410000000f2c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7609980
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC114270.15015808188720603TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT24300.026.94363812
CGTATGC25200.024.28498844
AGTCACT25250.024.14779328
CTCGTAT25000.023.66929242
TCGTATG26050.022.97436143
GCACACG29050.022.77040111
GTATGCC26900.022.75039745
CGTCTGA29650.021.93020416
CACGTCT30050.021.71316114
GTCTGAA30800.021.25747917
ACTGACC28050.021.01541332
ACGTCTG31200.020.62438215
AGCACAC32200.020.54286410
ACACGTC33350.019.49716813
ACCAATC29400.019.20860936
CACTGAC31700.019.0924931
CAGTCAC33050.018.92530427
GTCACTG32500.018.89943529
GACCAAT30400.018.79877935
TCACTGA32450.018.78988530
Produced by FastQC (version 0.11.2)