FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n01 mk270.3410000000f2a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n01 mk270.3410000000f2a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5020670
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC352390.7018784345515638TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGC42550.042.67214633
ACTTAGG42750.042.57776632
TTAGGCA43850.041.61230534
CACTTAG43950.041.21046431
TCACTTA44250.041.08360730
CACACGT49100.038.62927612
TAGGCAT47450.037.50686635
CTCGTAT49300.037.057842
GTATGCC49750.037.03954345
CGTATGC49950.037.02600544
AGTCACT49450.036.9453928
GTCACTT49650.036.75124729
TCGTATG50600.036.59483743
CACGTCT52450.036.24780714
GCACACG52700.036.1185511
GTCTGAA52500.036.0418617
CGTCTGA52600.035.9733416
AGCACAC54250.035.21100610
ACACGTC54350.035.1462213
ACGTCTG54150.035.0682815
Produced by FastQC (version 0.11.2)