FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n01 mk269.3410000000f286.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n01 mk269.3410000000f286.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4240859
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC1345593.1729185054254336TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT165500.043.27222412
AGTCACA163150.042.81984328
CGTCTGA166150.042.8185616
GCACACG167550.042.72935511
AGCACAC167900.042.72068410
ACGTCTG166800.042.69217315
GTCTGAA167100.042.5751317
GTCACAT164450.042.42662429
GTATGCC164350.042.42505645
CACGTCT168350.042.3792914
CGTATGC164650.042.29309544
CTCGTAT159950.042.28392842
CAGTCAC166300.042.27935827
GATCGGA171700.042.0318871
TCTGAAC169500.041.90592618
TCGTATG166100.041.896843
TCGGAAG172100.041.865583
ACACGTC170650.041.82129713
CTGAACT169850.041.7930819
AGAGCAC172100.041.7304048
Produced by FastQC (version 0.11.2)