FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n01 mk268.3410000000f26c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n01 mk268.3410000000f26c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16974320
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC3825942.2539577432262385TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT476850.042.71483212
GCACACG481450.042.38148511
TCACGCC462850.041.9943830
CGTCTGA483600.041.88616
TATCTCG446250.041.87758639
ACGTCTG484000.041.85138315
GTATGCC473700.041.75044645
GCCAATA463550.041.7465334
ACGCCAA464700.041.70615832
GTCACGC469850.041.59379629
CGTATGC476200.041.57777444
CACGTCT487550.041.56049714
CACGCCA465900.041.560131
GTCTGAA490650.041.33918417
AGCACAC498100.041.13192710
CGCCAAT471500.040.98059533
TCTGAAC494600.040.87711718
CTGAACT494750.040.8374419
CTCGTAT478350.040.7841542
GATCGGA509550.040.4849851
Produced by FastQC (version 0.11.2)