FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n01 mk267.3410000000f243.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n01 mk267.3410000000f243.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11223243
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1963371.7493784996012292TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT240650.042.17493412
GCTACAT224350.041.43827435
CTCGTAT233500.041.31762342
TACATCT221750.041.3153637
AGTCACG241850.041.12840328
TCGTATG239400.041.00421543
TCTCGTA226950.040.9139841
GTATGCC240700.040.86724545
ACACGTC248400.040.84097313
CACGTCT249450.040.68709614
CAGTCAC244950.040.67219527
ACGGCTA239650.040.6046832
TCTGAAC249400.040.56895418
CGTCTGA250650.040.48333416
TCGGAAG254300.040.412883
GTCTGAA251550.040.38321317
GGCTACA240400.040.32825534
ACGTCTG252200.040.24344615
AGAGCAC255350.040.0906378
AGCACAC254600.040.0231610