FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n01 mk266.3410000000f229.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n01 mk266.3410000000f229.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11358346
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC3379932.9757237541451897TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCT383600.043.29181732
TAGCTTA382700.043.2348934
TCACTAG386300.043.22220630
CACTAGC386200.043.08775731
CTAGCTT384150.043.03069733
CACACGT399700.042.72448312
GTCACTA392850.042.5645629
AGTCACT394850.042.37745328
AGCTTAT379150.042.32825535
CTCGTAT390450.042.10589242
CACGTCT405450.042.06863414
CTTATCT376150.042.01386337
TCGTATG397600.041.93156443
ACACGTC407050.041.92538513
GTATGCC399400.041.87270445
CAGTCAC403600.041.63710427
ACGTCTG409650.041.59887315
GTCTGAA410100.041.57517217
TATCTCG382400.041.5331239
CGTCTGA410450.041.5123116